pH Buffer Preparation

pH 4.01 Buffer Preparation.

1. – Accurately weigh out 8.954 g of Disodium hydrogen phosphate (Na2HPO4.12H2O) and 3.4023 g of potassium dihydrogen phosphate (KH2PO4).

2. – Dissolve both the above in distilled water and make up to 1L in a 1L volumetric flask. .

NOTE: self prepared solutions need to be stored in a fridge to prevent mold growth. However, keep in mind that the pH of your buffer is temperature dependent.

pH 6.86 Buffer Preparation.

1. – Accurately weigh out 10.212 g of Potassium hydrogen phthalate.

2. – Dissolve in distilled water and make up to 1L in a 1L volumetric flask.

NOTE: self prepared solutions need to be stored in a fridge to prevent mold growth. However, keep in mind that the pH of your buffer is temperature dependent.